Pharmacologically Active Saikosaponin in Bupleurum falcatum Detected by Competitive ELISA and Eastern Blotting Using Monoclonal Antibodies
This study showed the effective microanalytic methods for detection of saikosaponin by a competitive enzyme-linked immunosorbent assay (ELISA) using Wrench anti-saikosaponin a (SSa) monoclonal antibody (MAb).The results showed that the competitive ELISA had higher sensitivity than high performance liquid chromatography (HPLC) for the detection of s